Journal: Nature Communications

Article Title: OCT4 impedes cell fate redirection by the melanocyte lineage master regulator MITF in mouse ESCs

doi: 10.1038/s41467-017-01122-1

Figure Lengend Snippet: MITF efficiently transdifferentiates MEFs. a Bright field images of Mitf knock-in MEFs showing morphological changes at 12 days after Mitf induction by Dox supplementation and of untreated Mitf knock-in MEFs and primary mouse melanocytes. This was one of n = 3 experiments. b MITF and TYRP1 protein levels in Mitf knock-in MEFs at days 6 and 12 post Dox induction. This was one of n = 3 experiments. c Immunostaining of MITF (green) and TYRP1 (purple) in MEFs at day 6 post Dox induction with endogenous expression of mCherry (red). This was one of n = 3 experiments. d Hierarchical clustering of genes differentially expressed in MEFs before and after induction of Mitf expression (5864 genes) using Spearman correlation as a distance metric, Ward’s linkage, and per-row standardization (z score). e Spearman’s correlation was calculated over all exons that show differential ( > 4 fold change) expression between MEFs and melanocyte samples (45,155 exons)

Article Snippet: Hierarchical clustering was calculated over all differential genes (5864 genes) Matlab (version R2016b) clustergram command, using Spearman correlation as a distance metric, ward linkage, and per-row standardization (zscore) (Fig. ).

Techniques: Knock-In, Immunostaining, Expressing